Main Sector of relevance\IRC classification 10. Other Industrial Manufacturing Technologies

10.23 DNA isolation kit “DNA-BK”



Developers’ contact information

State Scientific Institution “The Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus”
220141, Minsk, Kuprevich St., 5/2
Tel./Fax: +375 (17) 267-87-61

Usanov Sergey Aleksandrovich
Теl.: +375 (17) 263-72-74; e-mail: usanov@iboch.bas-net.by

Summary

The kit “DNA-BK” is used for quick and effective isolation and clearance of DNA from buccal epithelium and whole blood samples.

Description

The method of isolation is based on specific reversible sorption of nucleic acids in the presence of chaotropic salts on the particles of silica gel. DNA preparations are suitable for the creation of genomic library, performing PCR, sequence analysis and can be used for gene diagnostic researches in research laboratories of the appropriate specialization, and also in clinical medicine.

Technology type

Technical advantages and economic benefits

- Effective isolation of DNA from buccal epithelium smears and whole blood samples (50-200 mcl);
- Isolation is held without toxic organic solvents (phenol, chloroform, isoamyl alcohol);
- There is no need in extra treatment with the help of proteinase and ribonuclease;
- Makes it possible to get 5-20 ng/mcl from buccal epithelium cells and 10-30 ng/mcl from whole blood;
- The possibility of protocol optimization for particular tasks;
- Isolation takes no less than 30 min.

Economic benefit manifests itself in substitution of imported analogs on the market of diagnostic test-systems for a cheaper kit with the same degree of safety. There are no domestic analogs.

Technology differentiation and uniqueness

Technology with know-how.

Context in which technology was identified

VIII National Exhibition of Republic of Belarus «Belarus Expo 2010» in Latvia; Youth Innovation Forum «INTRI» – 2010.

Technological keywords

Nucleic acids, DNA isolation, polymerase chain reaction, gene diagnostics.

Development Stage

Intellectual property rights

Range of applications

Clinical diagnostics. Molecular diagnostics. Sport genetics.

Classifier Used at the EU Innovation Relay Centres

Preferable Regions

Practical experience

Technology development.

Environmental impact

—

Type of collaboration sought

Terms and restrictions

License contract terms.

Support provided at transfer of the technology