Main Sector of relevance\IRC classification 3. Biological Sciences

3.20 Develop and introduce in health care practice the technology of determination of the tumor cells sensitivity of breast cancer in primary culture to anticancer chemotherapeutic drugs in order to select the optimal chemotherapy

Developers’ contact information

1) State Scientific Institution “The Institute of Biophysics and Cell Engineering”
220072, Minsk, Akademicheskaya St., 27
Теl.+375 (17) 284-17-49, Fax +375 (17) 284-23-59

Martinova Maria Alekseevna
Теl. +375 (17) 284-23-61; e-mail: martynova@ibp.org.by

Shukanova Natalia Andreevna  
Теl. +375 (29) 504-28-35; e-mail: natashu2006@yandex.ru

Putyrskij Leonid Alekseevich
Теl. +375 (17) 287-95-28

2) The Republican Research and Practical Center of Oncology and Medical Radiology named after N.N. Alexandrov.
223040, Minskij region, Lesnoj
Теl.: +375 (17) 287-95-05, Fax: +375 (17) 265-47-04; e-mail: oncobel@omr.med.by; URL: http://omr.med.by/

Summary

Definition technology  in vitro of chemotherapy drugs effect on metabolic processes in tumor cells of a breast cancer in primary culture.

Description

Conducting methods of primary culture of tumor cells in macro- and micro- quantities from operational and biopsy material of patients with the verified diagnosis of a breast cancer are developed.
The optimum biochemical indicators characterizing the chemotherapy drugs influence on activity of cellular enzymes, proliferation and initiation of apoptosis processes are chosen. The chemotherapy drugs are applied according to the report confirmed by Ministry of Health of the Republic of Belarus.
The tumor cells cultivation without chemotherapy drugs makes it possible to estimate the speed of cellular division which reflects the degree of malignant tumor’s development.

Photomicrographs of tumor cells which are obtained from trephine-biopsy of breast cancer
X100 increase. Pappenheim staining
 
cells primary culture
  
cells after 48 hours of cultivation in the presence of cytostatics group AC (doxorubicin + cyclophosphamide)

Technology type

Technical advantages and economic benefits

The technology is the basis for choosing the right strategy and tactics of neoadjuvant and adjuvant chemotherapy for breast cancer treatment, which significantly increases the efficiency of treatment, increases disease-free survival and overall survival and quality of life. It is expected to decrease the economic costs in the result of adequate selection of chemotherapy drugs for patients and to reduce the period of temporary incapacity.

Technology differentiation and uniqueness

New technology.

Context in which technology was identified

The rationalization proposal  № 120 from 29.07.2010 “The method of evaluating the effectiveness of chemotherapy drugs on tumor cells of breast cancer in primary culture using biochemical criteria” which is used in the department of oncomammology  since 17.06.2010 is implemented in the Republican Research and Practical Center of Oncology and Medical Radiology named after N.N. Alexandrov.

Technological keywords

Breast cancer, primary cultures of tumor cells, chemotherapy drugs, acetylcholinesterase, dehydrogenase.

Development Stage

Intellectual property rights

Range of applications

Medicine, oncology, oncomammalogy.

Classifier Used at the EU Innovation Relay Centres

Preferable Regions

Practical experience

Introduced and implemented.

Environmental impact

—

Type of collaboration sought

Terms and restrictions

—

Support provided at transfer of the technology